diff --git a/components/esp_driver_i2s/test_apps/.build-test-rules.yml b/components/esp_driver_i2s/test_apps/.build-test-rules.yml
index 09769644f68..a8f395c9c0f 100644
--- a/components/esp_driver_i2s/test_apps/.build-test-rules.yml
+++ b/components/esp_driver_i2s/test_apps/.build-test-rules.yml
@@ -3,6 +3,7 @@
 components/esp_driver_i2s/test_apps/i2s:
   disable:
     - if: SOC_I2S_SUPPORTED != 1
+    - if: CONFIG_NAME == "flash_enc" and SOC_FLASH_ENC_SUPPORTED != 1
   depends_components:
     - esp_driver_i2s
     - esp_driver_pcnt
diff --git a/components/esp_driver_i2s/test_apps/i2s/pytest_i2s.py b/components/esp_driver_i2s/test_apps/i2s/pytest_i2s.py
index 6a973559cd5..19f2cb6c950 100644
--- a/components/esp_driver_i2s/test_apps/i2s/pytest_i2s.py
+++ b/components/esp_driver_i2s/test_apps/i2s/pytest_i2s.py
@@ -76,3 +76,38 @@ def test_i2s_esp32c5_rev1(dut: Dut) -> None:
 )
 def test_i2s_psram(dut: Dut) -> None:
     dut.run_all_single_board_cases()
+
+
+@pytest.mark.flash_encryption_f4r8
+@pytest.mark.parametrize(
+    'config',
+    [
+        'flash_enc',
+    ],
+    indirect=True,
+)
+@idf_parametrize('target', ['esp32s3'], indirect=['target'])
+def test_i2s_with_flash_encryption_esp32s3_f4r8(dut: Dut) -> None:
+    dut.run_all_single_board_cases()
+
+
+@pytest.mark.flash_encryption
+@pytest.mark.parametrize(
+    'config',
+    [
+        'flash_enc',
+    ],
+    indirect=True,
+)
+@idf_parametrize(
+    'target',
+    soc_filtered_targets(
+        'SOC_I2S_SUPPORTED == 1 and '
+        'SOC_PSRAM_DMA_CAPABLE == 1 and '
+        'SOC_FLASH_ENC_SUPPORTED == 1 and '
+        'IDF_TARGET not in ["esp32s3"]'
+    ),
+    indirect=['target'],
+)
+def test_i2s_with_flash_encryption(dut: Dut) -> None:
+    dut.run_all_single_board_cases()
diff --git a/components/esp_driver_i2s/test_apps/i2s/sdkconfig.ci.flash_enc b/components/esp_driver_i2s/test_apps/i2s/sdkconfig.ci.flash_enc
new file mode 100644
index 00000000000..5be9651c381
--- /dev/null
+++ b/components/esp_driver_i2s/test_apps/i2s/sdkconfig.ci.flash_enc
@@ -0,0 +1,9 @@
+CONFIG_PARTITION_TABLE_OFFSET=0x9000
+CONFIG_SECURE_FLASH_ENC_ENABLED=y
+CONFIG_SECURE_FLASH_ENCRYPTION_MODE_DEVELOPMENT=y
+CONFIG_SECURE_BOOT_ALLOW_ROM_BASIC=y
+CONFIG_SECURE_BOOT_ALLOW_JTAG=y
+CONFIG_SECURE_FLASH_UART_BOOTLOADER_ALLOW_ENC=y
+CONFIG_SECURE_FLASH_UART_BOOTLOADER_ALLOW_DEC=y
+CONFIG_SECURE_FLASH_UART_BOOTLOADER_ALLOW_CACHE=y
+CONFIG_SECURE_FLASH_REQUIRE_ALREADY_ENABLED=y