diff --git a/.codespellrc b/.codespellrc
index 3e7c39a06b6..99b5f7ceec6 100644
--- a/.codespellrc
+++ b/.codespellrc
@@ -1,4 +1,4 @@
 [codespell]
 skip = build,*.yuv,components/fatfs/src/*,alice.txt,*.rgb,components/wpa_supplicant/*,components/esp_wifi/*,*.pem,components/newlib/COPYING.*
-ignore-words-list = ser,dout,rsource,fram,inout,shs,ans,aci,unstall,unstalling,hart,wheight,wel,ot,fane,assertIn,registr,oen,parms
+ignore-words-list = ser,dout,rsource,fram,inout,shs,ans,aci,unstall,unstalling,hart,wheight,wel,ot,fane,assertIn,registr,oen,parms,aCount
 write-changes = true
diff --git a/components/ieee802154/esp_ieee802154_multipan.c b/components/ieee802154/esp_ieee802154_multipan.c
index 3c15f8fc2a2..5a144cbd457 100644
--- a/components/ieee802154/esp_ieee802154_multipan.c
+++ b/components/ieee802154/esp_ieee802154_multipan.c
@@ -12,44 +12,46 @@
 #include "esp_ieee802154_pib.h"
 #include "esp_ieee802154_util.h"
 
+static uint8_t s_rx_when_idle_mask = 0;
+
 uint16_t esp_ieee802154_get_multipan_panid(esp_ieee802154_multipan_index_t index)
 {
-    assert(index < CONFIG_IEEE802154_INTERFACE_NUM);
+    IEEE802154_ASSERT(index < CONFIG_IEEE802154_INTERFACE_NUM);
     return ieee802154_ll_get_multipan_panid(index);
 }
 
 esp_err_t esp_ieee802154_set_multipan_panid(esp_ieee802154_multipan_index_t index, uint16_t panid)
 {
-    assert(index < CONFIG_IEEE802154_INTERFACE_NUM);
+    IEEE802154_ASSERT(index < CONFIG_IEEE802154_INTERFACE_NUM);
     ieee802154_ll_set_multipan_panid(index, panid);
     return ESP_OK;
 }
 
 uint16_t esp_ieee802154_get_multipan_short_address(esp_ieee802154_multipan_index_t index)
 {
-    assert(index < CONFIG_IEEE802154_INTERFACE_NUM);
+    IEEE802154_ASSERT(index < CONFIG_IEEE802154_INTERFACE_NUM);
     return ieee802154_ll_get_multipan_short_addr(index);
 }
 
 esp_err_t esp_ieee802154_set_multipan_short_address(esp_ieee802154_multipan_index_t index, uint16_t short_address)
 {
-    assert(index < CONFIG_IEEE802154_INTERFACE_NUM);
+    IEEE802154_ASSERT(index < CONFIG_IEEE802154_INTERFACE_NUM);
     ieee802154_ll_set_multipan_short_addr(index, short_address);
     return ESP_OK;
 }
 
 esp_err_t esp_ieee802154_get_multipan_extended_address(esp_ieee802154_multipan_index_t index, uint8_t *ext_addr)
 {
-    assert(index < CONFIG_IEEE802154_INTERFACE_NUM);
-    assert(ext_addr != NULL);
+    IEEE802154_ASSERT(index < CONFIG_IEEE802154_INTERFACE_NUM);
+    IEEE802154_ASSERT(ext_addr != NULL);
     ieee802154_ll_get_multipan_ext_addr(index, ext_addr);
     return ESP_OK;
 }
 
 esp_err_t esp_ieee802154_set_multipan_extended_address(esp_ieee802154_multipan_index_t index, const uint8_t *ext_addr)
 {
-    assert(index < CONFIG_IEEE802154_INTERFACE_NUM);
-    assert(ext_addr != NULL);
+    IEEE802154_ASSERT(index < CONFIG_IEEE802154_INTERFACE_NUM);
+    IEEE802154_ASSERT(ext_addr != NULL);
     ieee802154_ll_set_multipan_ext_addr(index, ext_addr);
     return ESP_OK;
 }
@@ -61,39 +63,79 @@ uint8_t esp_ieee802154_get_multipan_enable(void)
 
 esp_err_t esp_ieee802154_set_multipan_enable(uint8_t mask)
 {
-    assert(mask < (1 << CONFIG_IEEE802154_INTERFACE_NUM));
+    IEEE802154_ASSERT(mask < (1 << CONFIG_IEEE802154_INTERFACE_NUM));
     ieee802154_ll_set_multipan_enable_mask(mask);
     return ESP_OK;
 }
 
 esp_ieee802154_pending_mode_t esp_ieee802154_multipan_get_pending_mode(esp_ieee802154_multipan_index_t index)
 {
-    assert(index < CONFIG_IEEE802154_INTERFACE_NUM);
+    IEEE802154_ASSERT(index < CONFIG_IEEE802154_INTERFACE_NUM);
     return ieee802154_pib_get_pending_mode(index);
 }
 
 esp_err_t esp_ieee802154_multipan_set_pending_mode(esp_ieee802154_multipan_index_t inf_index, esp_ieee802154_pending_mode_t pending_mode)
 {
-    assert(inf_index < CONFIG_IEEE802154_INTERFACE_NUM);
+    IEEE802154_ASSERT(inf_index < CONFIG_IEEE802154_INTERFACE_NUM);
     ieee802154_pib_set_pending_mode(inf_index, pending_mode);
     return ESP_OK;
 }
 
 esp_err_t esp_ieee802154_multipan_add_pending_addr(esp_ieee802154_multipan_index_t inf_index, const uint8_t *addr, bool is_short)
 {
-    assert(inf_index < CONFIG_IEEE802154_INTERFACE_NUM);
+    IEEE802154_ASSERT(inf_index < CONFIG_IEEE802154_INTERFACE_NUM);
     return ieee802154_add_pending_addr(inf_index, addr, is_short);
 }
 
 esp_err_t esp_ieee802154_multipan_clear_pending_addr(esp_ieee802154_multipan_index_t inf_index, const uint8_t *addr, bool is_short)
 {
-    assert(inf_index < CONFIG_IEEE802154_INTERFACE_NUM);
+    IEEE802154_ASSERT(inf_index < CONFIG_IEEE802154_INTERFACE_NUM);
     return ieee802154_clear_pending_addr(inf_index, addr, is_short);
 }
 
 esp_err_t esp_ieee802154_multipan_reset_pending_table(esp_ieee802154_multipan_index_t inf_index, bool is_short)
 {
-    assert(inf_index < CONFIG_IEEE802154_INTERFACE_NUM);
+    IEEE802154_ASSERT(inf_index < CONFIG_IEEE802154_INTERFACE_NUM);
     ieee802154_reset_pending_table(inf_index, is_short);
     return ESP_OK;
 }
+
+esp_err_t esp_ieee802154_multipan_sleep(int8_t idx)
+{
+    IEEE802154_ASSERT(idx >= 0 && idx < CONFIG_IEEE802154_INTERFACE_NUM);
+    uint8_t enable_mask = ieee802154_ll_get_multipan_enable_mask();
+    enable_mask &= ~(1 << idx);
+    IEEE802154_ASSERT(enable_mask < (1 << CONFIG_IEEE802154_INTERFACE_NUM));
+    ieee802154_ll_set_multipan_enable_mask(enable_mask);
+    if (enable_mask == 0) {
+        return ieee802154_sleep();
+    }
+    return ESP_OK;
+}
+
+esp_err_t esp_ieee802154_multipan_receive(int8_t idx)
+{
+    IEEE802154_ASSERT(idx >= 0 && idx < CONFIG_IEEE802154_INTERFACE_NUM);
+    uint8_t enable_mask = ieee802154_ll_get_multipan_enable_mask();
+    enable_mask |= (1 << idx);
+    IEEE802154_ASSERT(enable_mask < (1 << CONFIG_IEEE802154_INTERFACE_NUM));
+    ieee802154_ll_set_multipan_enable_mask(enable_mask);
+    return ieee802154_receive();
+}
+
+esp_err_t esp_ieee802154_multipan_set_rx_when_idle(int8_t idx, bool enable)
+{
+    IEEE802154_ASSERT(idx >= 0 && idx < CONFIG_IEEE802154_INTERFACE_NUM);
+    if (enable) {
+        s_rx_when_idle_mask |= (1 << idx);
+        ieee802154_pib_set_rx_when_idle(true);
+    } else {
+        s_rx_when_idle_mask &= ~(1 << idx);
+        if (s_rx_when_idle_mask == 0) {
+            ieee802154_pib_set_rx_when_idle(false);
+        }
+    }
+    return ESP_OK;
+}
+
+// Note: multipan receive_at is not supported yet.
diff --git a/components/ieee802154/include/esp_ieee802154_multipan.h b/components/ieee802154/include/esp_ieee802154_multipan.h
index f49da2a4b87..58766f8585a 100644
--- a/components/ieee802154/include/esp_ieee802154_multipan.h
+++ b/components/ieee802154/include/esp_ieee802154_multipan.h
@@ -182,6 +182,43 @@ esp_ieee802154_pending_mode_t esp_ieee802154_multipan_get_pending_mode(esp_ieee8
  */
 esp_err_t esp_ieee802154_multipan_set_pending_mode(esp_ieee802154_multipan_index_t inf_index, esp_ieee802154_pending_mode_t pending_mode);
 
+/**
+ * @brief  Set the IEEE 802.15.4 Radio to sleep state for the specified multipan interface.
+ *
+ * @param[in]  idx  Index of the multipan interface.
+ *
+ * @return
+ *      - ESP_OK on success.
+ *      - ESP_FAIL on failure.
+ */
+esp_err_t esp_ieee802154_multipan_sleep(int8_t idx);
+
+/**
+ * @brief  Set the IEEE 802.15.4 Radio to receive state for the specified multipan interface.
+ *
+ * @param[in]  idx  Index of the multipan interface.
+ *
+ * @return
+ *      - ESP_OK on success.
+ *      - ESP_FAIL on failure.
+ */
+esp_err_t esp_ieee802154_multipan_receive(int8_t idx);
+
+/**
+ * @brief  Set the RxOnWhenIdle mode for the specified multipan interface.
+ *
+ * Each interface owns one bit in an internal mask. The hardware RxOnWhenIdle
+ * setting is enabled when any interface requests it, and is disabled only
+ * when all interface bits are cleared.
+ *
+ * @param[in]  idx     Index of the multipan interface.
+ * @param[in]  enable  True to enable RxOnWhenIdle for this interface.
+ *
+ * @return
+ *      - ESP_OK on success.
+ */
+esp_err_t esp_ieee802154_multipan_set_rx_when_idle(int8_t idx, bool enable);
+
 #ifdef __cplusplus
 }
 #endif
diff --git a/components/openthread/Kconfig b/components/openthread/Kconfig
index 8e0d219e663..04a48aabe3b 100644
--- a/components/openthread/Kconfig
+++ b/components/openthread/Kconfig
@@ -517,6 +517,22 @@ menu "OpenThread"
                 OpenThread esp_netif, attaches the esp_netif glue, and enables
                 lwIP-specific DNS/netif handling.
 
+        config OPENTHREAD_MULTIPAN_RCP_ENABLE
+            bool "Enable Multipan RCP"
+            depends on (OPENTHREAD_RADIO && IEEE802154_MULTI_PAN_ENABLE)
+            default n
+            help
+                Enable MULTI-PAN RCP support. This also enables multiple OpenThread
+                instances. Currently only radio devices are supported.
+
+        config OPENTHREAD_MULTIPLE_INTERFACES_COUNT
+            int "The number of multiple interfaces"
+            depends on OPENTHREAD_MULTIPAN_RCP_ENABLE
+            default 2
+            range 2 3
+            help
+                Number of multiple interfaces.
+
     endmenu
 
     menu "Thread Log"
diff --git a/components/openthread/openthread b/components/openthread/openthread
index b678a4f63b6..43cc05a9bcf 160000
--- a/components/openthread/openthread
+++ b/components/openthread/openthread
@@ -1 +1 @@
-Subproject commit b678a4f63b6f9397d1a0fa8f31e5b8e0271a4d00
+Subproject commit 43cc05a9bcf780bd758bad53d897e2d88cf8cb75
diff --git a/components/openthread/private_include/esp_openthread_common_macro.h b/components/openthread/private_include/esp_openthread_common_macro.h
index 8595bc7684b..4893e7fb3cc 100644
--- a/components/openthread/private_include/esp_openthread_common_macro.h
+++ b/components/openthread/private_include/esp_openthread_common_macro.h
@@ -42,3 +42,9 @@
 #else
 #define ESP_OPENTHREAD_ASSERT(a) assert(a)
 #endif
+
+#if CONFIG_OPENTHREAD_MULTIPAN_RCP_ENABLE
+#define OT_INSTANCE_COUNT CONFIG_OPENTHREAD_MULTIPLE_INTERFACES_COUNT
+#else
+#define OT_INSTANCE_COUNT 1
+#endif
diff --git a/components/openthread/private_include/esp_openthread_ncp.h b/components/openthread/private_include/esp_openthread_ncp.h
index 2d529dd7704..05026cd766a 100644
--- a/components/openthread/private_include/esp_openthread_ncp.h
+++ b/components/openthread/private_include/esp_openthread_ncp.h
@@ -1,5 +1,5 @@
 /*
- * SPDX-FileCopyrightText: 2024-2025 Espressif Systems (Shanghai) CO LTD
+ * SPDX-FileCopyrightText: 2024-2026 Espressif Systems (Shanghai) CO LTD
  *
  * SPDX-License-Identifier: Apache-2.0
  */
@@ -10,8 +10,21 @@
 extern "C" {
 #endif
 
+/**
+ * @brief This function initializes the NCP for a single instance
+ *
+ * @param[in]    aInstance   The OpenThread instance.
+ */
 void otAppNcpInit(otInstance *aInstance);
 
+/**
+ * @brief This function initializes the NCP for multiple instances
+ *
+ * @param[in]    aInstances   The array of OpenThread instances.
+ * @param[in]    aCount       The number of instances.
+ */
+void otAppNcpInitMulti(otInstance **aInstances, uint8_t aCount);
+
 #if CONFIG_OPENTHREAD_RCP_SPINEL_CONSOLE
 esp_err_t esp_console_redirect_to_otlog(void);
 #endif // CONFIG_OPENTHREAD_RCP_SPINEL_CONSOLE
diff --git a/components/openthread/private_include/esp_openthread_platform.h b/components/openthread/private_include/esp_openthread_platform.h
index 582599f5d7f..faa78ec1d65 100644
--- a/components/openthread/private_include/esp_openthread_platform.h
+++ b/components/openthread/private_include/esp_openthread_platform.h
@@ -1,5 +1,5 @@
 /*
- * SPDX-FileCopyrightText: 2021-2025 Espressif Systems (Shanghai) CO LTD
+ * SPDX-FileCopyrightText: 2021-2026 Espressif Systems (Shanghai) CO LTD
  *
  * SPDX-License-Identifier: Apache-2.0
  */
@@ -155,6 +155,37 @@ void esp_openthread_set_storage_name(const char *name);
  */
 uint32_t esp_openthread_get_alloc_caps(void);
 
+/**
+ * @brief This function gets the index of the instance
+ *
+ * @param[in]    instance   The OpenThread instance.
+ *
+ * @note Invalid input is logged and asserted.
+ *
+ * @return
+ *      - The index of the instance
+ */
+int8_t esp_openthread_get_idx_from_instance(otInstance *instance);
+
+/**
+ * @brief This function gets the instance from the index
+ *
+ * @param[in]    idx   The index of the instance.
+ *
+ * @note Invalid index is logged and asserted. A valid index always has an instance.
+ *
+ * @return
+ *      - The instance
+ */
+otInstance *esp_openthread_get_instance_from_idx(int8_t idx);
+
+/**
+ * @brief This function sets the active instance
+ *
+ * @param[in]    instance   The OpenThread instance.
+ */
+void esp_openthread_set_active_instance(otInstance *instance);
+
 #ifdef __cplusplus
 } // end of extern "C"
 #endif
diff --git a/components/openthread/private_include/openthread-core-esp32x-radio-config.h b/components/openthread/private_include/openthread-core-esp32x-radio-config.h
index 3df6677e2e1..133c7b7c215 100644
--- a/components/openthread/private_include/openthread-core-esp32x-radio-config.h
+++ b/components/openthread/private_include/openthread-core-esp32x-radio-config.h
@@ -165,6 +165,62 @@
 #define OPENTHREAD_CONFIG_TIME_SYNC_ENABLE 0
 #endif
 
+/**
+ * @def OPENTHREAD_CONFIG_MULTIPLE_INSTANCE_ENABLE
+ *
+ * Define to 1 to enable multiple instance support.
+ */
+#ifdef OPENTHREAD_CONFIG_MULTIPLE_INSTANCE_ENABLE
+#error `OPENTHREAD_CONFIG_MULTIPLE_INSTANCE_ENABLE` is redefined.
+#endif
+#if CONFIG_OPENTHREAD_MULTIPAN_RCP_ENABLE
+#define OPENTHREAD_CONFIG_MULTIPLE_INSTANCE_ENABLE 1
+#else
+#define OPENTHREAD_CONFIG_MULTIPLE_INSTANCE_ENABLE 0
+#endif
+
+/**
+ * @def OPENTHREAD_CONFIG_MULTIPAN_RCP_ENABLE
+ *
+ * Define to 1 to enable multipan RCP support.
+ */
+#ifdef OPENTHREAD_CONFIG_MULTIPAN_RCP_ENABLE
+#error `OPENTHREAD_CONFIG_MULTIPAN_RCP_ENABLE` is redefined.
+#endif
+#if CONFIG_OPENTHREAD_MULTIPAN_RCP_ENABLE
+#define OPENTHREAD_CONFIG_MULTIPAN_RCP_ENABLE 1
+#else
+#define OPENTHREAD_CONFIG_MULTIPAN_RCP_ENABLE 0
+#endif
+
+/**
+ * @def OPENTHREAD_CONFIG_MULTIPLE_STATIC_INSTANCE_ENABLE
+ *
+ * Define to 1 to enable multiple static instance support.
+ */
+#ifdef OPENTHREAD_CONFIG_MULTIPLE_STATIC_INSTANCE_ENABLE
+#error `OPENTHREAD_CONFIG_MULTIPLE_STATIC_INSTANCE_ENABLE` is redefined.
+#endif
+#if CONFIG_OPENTHREAD_MULTIPAN_RCP_ENABLE
+#define OPENTHREAD_CONFIG_MULTIPLE_STATIC_INSTANCE_ENABLE 1
+#else
+#define OPENTHREAD_CONFIG_MULTIPLE_STATIC_INSTANCE_ENABLE 0
+#endif
+
+/**
+ * @def OPENTHREAD_SPINEL_CONFIG_BROADCAST_IID
+ *
+ * Define broadcast IID for spinel frames dedicated to all hosts in multipan configuration.
+ * Host interfaces use IID 0 .. COUNT-1; broadcast takes the next slot.
+ *
+ */
+#ifdef OPENTHREAD_SPINEL_CONFIG_BROADCAST_IID
+#error `OPENTHREAD_SPINEL_CONFIG_BROADCAST_IID` is redefined.
+#endif
+#if CONFIG_OPENTHREAD_MULTIPAN_RCP_ENABLE
+#define OPENTHREAD_SPINEL_CONFIG_BROADCAST_IID SPINEL_HEADER_IID(CONFIG_OPENTHREAD_MULTIPLE_INTERFACES_COUNT)
+#endif
+
 /*----The following options set fixed default values but can be overridden by the user header file.----*/
 
 #if CONFIG_OPENTHREAD_LINK_METRICS
diff --git a/components/openthread/sbom_openthread.yml b/components/openthread/sbom_openthread.yml
index 6d40f289e71..52b620afc19 100644
--- a/components/openthread/sbom_openthread.yml
+++ b/components/openthread/sbom_openthread.yml
@@ -5,7 +5,7 @@ supplier: 'Organization: Espressif Systems (Shanghai) CO LTD'
 originator: 'Organization: Google LLC'
 description: OpenThread released by Google is an open-source implementation of the Thread networking
 url: https://github.com/espressif/openthread
-hash: b678a4f63b6f9397d1a0fa8f31e5b8e0271a4d00
+hash: 43cc05a9bcf780bd758bad53d897e2d88cf8cb75
 cve-exclude-list:
   - cve: CVE-2026-8369
     reason: We use Espressif’s NAT64 implementation and hence this CVE from the upstream NAT64 implementation is not applicable.
diff --git a/components/openthread/src/multiple_instances/esp_openthread_instance.cpp b/components/openthread/src/multiple_instances/esp_openthread_instance.cpp
index c52daf509c7..e19e5f7b44e 100644
--- a/components/openthread/src/multiple_instances/esp_openthread_instance.cpp
+++ b/components/openthread/src/multiple_instances/esp_openthread_instance.cpp
@@ -5,16 +5,27 @@
  */
 
 #include "sdkconfig.h"
-#include "core/instance/instance.hpp"
+#include <assert.h>
+#include "esp_log.h"
 #include "esp_openthread.h"
+#include "esp_openthread_common_macro.h"
 #include "esp_openthread_instance.h"
 #include "esp_openthread_ncp.h"
+#include "esp_openthread_platform.h"
 #include "openthread/instance.h"
 #include "openthread/tasklet.h"
 
+static otInstance *s_active_instance = NULL;
+static otInstance *s_instances[OT_INSTANCE_COUNT] = {NULL};
+
 bool esp_openthread_instances_init(void)
 {
-    return otInstanceInitSingle() != NULL;
+    for (int i = 0; i < OT_INSTANCE_COUNT; i++) {
+        s_instances[i] = otInstanceInitMultiple(i);
+        assert(s_instances[i]);
+    }
+    esp_openthread_set_active_instance(s_instances[0]);
+    return true;
 }
 
 void esp_openthread_instances_deinit(otInstance *instance)
@@ -24,24 +35,79 @@ void esp_openthread_instances_deinit(otInstance *instance)
 
 bool esp_openthread_tasklets_are_pending(otInstance *instance)
 {
-    return otTaskletsArePending(instance);
+    (void)instance;
+
+    for (int idx = 0; idx < OT_INSTANCE_COUNT; idx++) {
+        if (otTaskletsArePending(esp_openthread_get_instance_from_idx(idx))) {
+            return true;
+        }
+    }
+    return false;
 }
 
 void esp_openthread_tasklets_process(otInstance *instance)
 {
-    while (otTaskletsArePending(instance)) {
-        otTaskletsProcess(instance);
+    (void)instance;
+
+    for (int idx = 0; idx < OT_INSTANCE_COUNT; idx++) {
+        otInstance *inst = esp_openthread_get_instance_from_idx(idx);
+        while (otTaskletsArePending(inst)) {
+            otTaskletsProcess(inst);
+        }
     }
 }
 
 #if CONFIG_OPENTHREAD_RADIO
 void esp_openthread_ncp_app_init(otInstance *instance)
 {
-    otAppNcpInit(instance);
+    (void)instance;
+
+    otInstance *instances[OT_INSTANCE_COUNT] = {nullptr};
+    for (int idx = 0; idx < OT_INSTANCE_COUNT; idx++) {
+        instances[idx] = esp_openthread_get_instance_from_idx(idx);
+    }
+    otAppNcpInitMulti(instances, OT_INSTANCE_COUNT);
 }
 #endif
 
 otInstance *esp_openthread_get_instance(void)
 {
-    return (otInstance *)&ot::Instance::Get();
+    return s_active_instance;
+}
+
+int8_t esp_openthread_get_idx_from_instance(otInstance *instance)
+{
+    if (instance == nullptr) {
+        ESP_LOGE(OT_PLAT_LOG_TAG, "Instance is NULL");
+        assert(instance != nullptr);
+    }
+
+    uint8_t idx = otInstanceGetIndex(instance);
+    if (idx >= OT_INSTANCE_COUNT) {
+        ESP_LOGE(OT_PLAT_LOG_TAG, "Index %u is out of range", idx);
+        assert(idx < OT_INSTANCE_COUNT);
+    }
+
+    return static_cast<int8_t>(idx);
+}
+
+otInstance *esp_openthread_get_instance_from_idx(int8_t idx)
+{
+    if (idx < 0 || idx >= OT_INSTANCE_COUNT) {
+        ESP_LOGE(OT_PLAT_LOG_TAG, "Index %d is out of range", idx);
+        assert(idx >= 0 && idx < OT_INSTANCE_COUNT);
+    }
+
+    otInstance *instance = otInstanceGetInstance(static_cast<uint8_t>(idx));
+    if (instance == nullptr) {
+        ESP_LOGE(OT_PLAT_LOG_TAG, "No instance for index %d", idx);
+        assert(instance != nullptr);
+    }
+
+    return instance;
+}
+
+void esp_openthread_set_active_instance(otInstance *instance)
+{
+    s_active_instance = instance;
 }
diff --git a/components/openthread/src/multiple_instances/port/esp_openthread_alarm.c b/components/openthread/src/multiple_instances/port/esp_openthread_alarm.c
index 091e557211a..82f3f4ba562 100644
--- a/components/openthread/src/multiple_instances/port/esp_openthread_alarm.c
+++ b/components/openthread/src/multiple_instances/port/esp_openthread_alarm.c
@@ -11,6 +11,7 @@
 #include <sys/time.h>
 
 #include "esp_log.h"
+#include "esp_openthread.h"
 #include "esp_openthread_common_macro.h"
 #include "esp_openthread_platform.h"
 #include "esp_timer.h"
@@ -23,10 +24,15 @@
 #include "openthread/platform/radio.h"
 #include "openthread/platform/time.h"
 
-static uint32_t s_alarm_ms = 0;
-static bool s_is_ms_running = false;
-static uint32_t s_alarm_us = 0;
-static bool s_is_us_running = false;
+typedef struct {
+    uint32_t alarm_ms;
+    bool is_ms_running;
+    uint32_t alarm_us;
+    bool is_us_running;
+    otInstance *instance;
+} esp_openthread_alarm_t;
+
+static esp_openthread_alarm_t s_alarms[OT_INSTANCE_COUNT];
 static const char *alarm_workflow = "alarm";
 
 static inline bool is_expired(uint32_t target, uint32_t now)
@@ -46,19 +52,19 @@ uint64_t otPlatTimeGet(void)
 
 void otPlatAlarmMilliStartAt(otInstance *aInstance, uint32_t aT0, uint32_t aDt)
 {
-    OT_UNUSED_VARIABLE(aInstance);
+    int8_t idx = esp_openthread_get_idx_from_instance(aInstance);
 
-    s_alarm_ms = aT0 + aDt;
-    s_is_ms_running = true;
+    s_alarms[idx].alarm_ms = aT0 + aDt;
+    s_alarms[idx].is_ms_running = true;
 
     ESP_LOGD(OT_PLAT_LOG_TAG, "Millisecond timer alarm start running, t0=%"PRIu32", dt=%"PRIu32"", aT0, aDt);
 }
 
 void otPlatAlarmMilliStop(otInstance *aInstance)
 {
-    OT_UNUSED_VARIABLE(aInstance);
+    int8_t idx = esp_openthread_get_idx_from_instance(aInstance);
 
-    s_is_ms_running = false;
+    s_alarms[idx].is_ms_running = false;
 }
 
 uint32_t inline otPlatAlarmMilliGetNow(void)
@@ -68,18 +74,19 @@ uint32_t inline otPlatAlarmMilliGetNow(void)
 
 void otPlatAlarmMicroStartAt(otInstance *aInstance, uint32_t aT0, uint32_t aDt)
 {
-    OT_UNUSED_VARIABLE(aInstance);
+    int8_t idx = esp_openthread_get_idx_from_instance(aInstance);
 
-    s_alarm_us = aT0 + aDt;
-    s_is_us_running = true;
+    s_alarms[idx].alarm_us = aT0 + aDt;
+    s_alarms[idx].is_us_running = true;
 
     ESP_LOGD(OT_PLAT_LOG_TAG, "Microsecond timer alarm start running, t0=%"PRIu32", dt=%"PRIu32"", aT0, aDt);
 }
 
 void otPlatAlarmMicroStop(otInstance *aInstance)
 {
-    OT_UNUSED_VARIABLE(aInstance);
-    s_is_us_running = false;
+    int8_t idx = esp_openthread_get_idx_from_instance(aInstance);
+
+    s_alarms[idx].is_us_running = false;
 }
 
 uint32_t inline otPlatAlarmMicroGetNow(void)
@@ -89,6 +96,9 @@ uint32_t inline otPlatAlarmMicroGetNow(void)
 
 esp_err_t esp_openthread_alarm_init(void)
 {
+    for (int idx = 0; idx < OT_INSTANCE_COUNT; idx++) {
+        memset((void *)(&s_alarms[idx]), 0, sizeof(esp_openthread_alarm_t));
+    }
     return esp_openthread_platform_workflow_register(&esp_openthread_alarm_update, &esp_openthread_alarm_process,
                                                      alarm_workflow);
 }
@@ -103,19 +113,22 @@ void esp_openthread_alarm_update(esp_openthread_mainloop_context_t *mainloop)
     struct timeval *timeout = &mainloop->timeout;
     int64_t remain_min_time_us = INT64_MAX;
     int64_t remaining_us = 0;
-    if (s_is_ms_running) {
-        remaining_us = calculate_duration(s_alarm_ms, otPlatAlarmMilliGetNow()) * US_PER_MS;
-        if (remain_min_time_us > remaining_us) {
-            remain_min_time_us = remaining_us;
+
+    for (int idx = 0; idx < OT_INSTANCE_COUNT; idx++) {
+        if (s_alarms[idx].is_ms_running) {
+            remaining_us = calculate_duration(s_alarms[idx].alarm_ms, otPlatAlarmMilliGetNow()) * US_PER_MS;
+            if (remain_min_time_us > remaining_us) {
+                remain_min_time_us = remaining_us;
+            }
+        }
+        if (s_alarms[idx].is_us_running) {
+            remaining_us = calculate_duration(s_alarms[idx].alarm_us, otPlatAlarmMicroGetNow());
+            if (remain_min_time_us > remaining_us) {
+                remain_min_time_us = remaining_us;
+            }
         }
     }
-    if (s_is_us_running) {
-        remaining_us = calculate_duration(s_alarm_us, otPlatAlarmMicroGetNow());
-        if (remain_min_time_us > remaining_us) {
-            remain_min_time_us = remaining_us;
-        }
-    }
-    if (remain_min_time_us > 0) {
+    if (remain_min_time_us != INT64_MAX && remain_min_time_us > 0) {
         timeout->tv_sec = remain_min_time_us / US_PER_S;
         timeout->tv_usec = remain_min_time_us % US_PER_S;
     } else {
@@ -126,24 +139,29 @@ void esp_openthread_alarm_update(esp_openthread_mainloop_context_t *mainloop)
 
 esp_err_t esp_openthread_alarm_process(otInstance *aInstance, const esp_openthread_mainloop_context_t *mainloop)
 {
-    if (s_is_ms_running && is_expired(s_alarm_ms, otPlatAlarmMilliGetNow())) {
-        s_is_ms_running = false;
+    OT_UNUSED_VARIABLE(aInstance);
+    OT_UNUSED_VARIABLE(mainloop);
+    for (int idx = 0; idx < OT_INSTANCE_COUNT; idx++) {
+        otInstance *instance = esp_openthread_get_instance_from_idx(idx);
+        if (s_alarms[idx].is_ms_running && is_expired(s_alarms[idx].alarm_ms, otPlatAlarmMilliGetNow())) {
+            s_alarms[idx].is_ms_running = false;
 
 #if OPENTHREAD_CONFIG_DIAG_ENABLE
-        if (otPlatDiagModeGet()) {
-            otPlatDiagAlarmFired(aInstance);
-        } else
+            if (otPlatDiagModeGet()) {
+                otPlatDiagAlarmFired(instance);
+            } else
 #endif
-        {
-            otPlatAlarmMilliFired(aInstance);
-        }
+            {
+                otPlatAlarmMilliFired(instance);
+            }
 
-        ESP_LOGD(OT_PLAT_LOG_TAG, "Millisecond timer alarm fired");
-    }
-    if (s_is_us_running && is_expired(s_alarm_us, otPlatAlarmMicroGetNow())) {
-        s_is_us_running = false;
-        otPlatAlarmMicroFired(aInstance);
-        ESP_LOGD(OT_PLAT_LOG_TAG, "Microsecond timer alarm fired");
+            ESP_LOGD(OT_PLAT_LOG_TAG, "Millisecond timer alarm fired");
+        }
+        if (s_alarms[idx].is_us_running && is_expired(s_alarms[idx].alarm_us, otPlatAlarmMicroGetNow())) {
+            s_alarms[idx].is_us_running = false;
+            otPlatAlarmMicroFired(instance);
+            ESP_LOGD(OT_PLAT_LOG_TAG, "Microsecond timer alarm fired");
+        }
     }
     return ESP_OK;
 }
diff --git a/components/openthread/src/multiple_instances/port/esp_openthread_radio.c b/components/openthread/src/multiple_instances/port/esp_openthread_radio.c
index e99ea7034df..45c38754edd 100644
--- a/components/openthread/src/multiple_instances/port/esp_openthread_radio.c
+++ b/components/openthread/src/multiple_instances/port/esp_openthread_radio.c
@@ -10,7 +10,10 @@
 #include "sdkconfig.h"
 #include "esp_check.h"
 #include "esp_ieee802154.h"
+#include "esp_ieee802154_multipan.h"
+#include "esp_ieee802154_types.h"
 #include "esp_mac.h"
+#include "esp_openthread.h"
 #include "esp_openthread_common.h"
 #include "esp_openthread_common_macro.h"
 #include "esp_openthread_platform.h"
@@ -26,6 +29,7 @@
 #include "openthread/error.h"
 #include "openthread/link.h"
 #include "openthread/platform/diag.h"
+#include "openthread/platform/multipan.h"
 #include "openthread/platform/radio.h"
 #include "openthread/platform/time.h"
 #include "utils/link_metrics.h"
@@ -51,11 +55,20 @@ typedef struct {
     uint8_t psdu[OT_RADIO_FRAME_MAX_SIZE];
 } esp_openthread_radio_tx_psdu;
 
-static otRadioFrame s_transmit_frame;
+typedef struct esp_openthread_radio_transmit_context {
+    int8_t index;
+    otRadioFrame *aFrame;
+} esp_openthread_radio_transmit_context_t;
 
-static esp_openthread_radio_tx_psdu s_transmit_psdu;
+typedef struct esp_openthread_radio_receive_context {
+    int8_t index;
+    otRadioFrame frame;
+} esp_openthread_radio_receive_context_t;
+
+static otRadioFrame s_transmit_frame[OT_INSTANCE_COUNT];
+static esp_openthread_radio_tx_psdu s_transmit_psdu[OT_INSTANCE_COUNT];
 static uint8_t *s_enhack;
-static otRadioFrame s_receive_frame[CONFIG_IEEE802154_RX_BUFFER_SIZE];
+static esp_openthread_radio_receive_context_t s_receive_frame[CONFIG_IEEE802154_RX_BUFFER_SIZE];
 static otRadioFrame s_ack_frame;
 static int s_ed_power;
 static esp_ieee802154_tx_error_t s_tx_error;
@@ -68,7 +81,7 @@ static bool s_diag_mode = false;
 #endif
 
 #if OPENTHREAD_CONFIG_MAC_HEADER_IE_SUPPORT
-static otRadioIeInfo s_transmit_ie_info;
+static otRadioIeInfo s_transmit_ie_info[OT_INSTANCE_COUNT];
 #endif // OPENTHREAD_CONFIG_MAC_HEADER_IE_SUPPORT
 
 #if OPENTHREAD_CONFIG_MAC_CSL_RECEIVER_ENABLE
@@ -77,44 +90,107 @@ static uint32_t s_csl_sample_time;
 #endif // OPENTHREAD_CONFIG_MAC_CSL_RECEIVER_ENABLE
 
 #if OPENTHREAD_CONFIG_THREAD_VERSION >= OT_THREAD_VERSION_1_2
-static uint32_t s_mac_frame_counter;
-static uint8_t s_key_id;
-static struct otMacKeyMaterial s_previous_key;
-static struct otMacKeyMaterial s_current_key;
-static struct otMacKeyMaterial s_next_key;
+typedef struct {
+    uint32_t mac_frame_counter;
+    uint8_t key_id;
+    struct otMacKeyMaterial previous_key;
+    struct otMacKeyMaterial current_key;
+    struct otMacKeyMaterial next_key;
 #if OPENTHREAD_CONFIG_PLATFORM_KEY_REFERENCES_ENABLE
-static uint8_t s_pervious_key_bytes[16];
-static uint8_t s_current_key_bytes[16];
-static uint8_t s_next_key_bytes[16];
+    uint8_t previous_key_bytes[16];
+    uint8_t current_key_bytes[16];
+    uint8_t next_key_bytes[16];
 #endif
-static bool s_with_security_enh_ack = false;
-static uint32_t s_ack_frame_counter;
-static uint8_t s_ack_key_id;
-static uint8_t s_security_key[16];
-static uint8_t s_security_addr[8];
+    bool with_security_enh_ack;
+    uint32_t ack_frame_counter;
+    uint8_t ack_key_id;
+    uint8_t security_key[16];
+    uint8_t security_addr[8];
+} esp_openthread_radio_security_ctx_t;
 
-static void ot_set_security_key_from_key_material(struct otMacKeyMaterial a_key_material)
+static esp_openthread_radio_security_ctx_t s_sec_ctx[OT_INSTANCE_COUNT];
+#endif // OPENTHREAD_CONFIG_THREAD_VERSION >= OT_THREAD_VERSION_1_2
+
+/* Head is the in-flight TX; remaining entries are pending.
+ * Only touched in OpenThread task context (ISR only reads head/index). */
+typedef struct {
+    uint8_t head;
+    uint8_t tail;
+    uint8_t used;
+} esp_openthread_tx_queue_info_t;
+
+static esp_openthread_radio_transmit_context_t s_tx_queue[OT_INSTANCE_COUNT];
+static esp_openthread_tx_queue_info_t s_tx_queue_info = {.head = 0, .tail = 0, .used = 0};
+
+#if OPENTHREAD_CONFIG_THREAD_VERSION >= OT_THREAD_VERSION_1_2
+static void ot_set_security_key_from_key_material(int8_t idx, struct otMacKeyMaterial a_key_material)
 {
+    if (idx < 0 || idx >= OT_INSTANCE_COUNT) {
+        return;
+    }
 #if OPENTHREAD_CONFIG_PLATFORM_KEY_REFERENCES_ENABLE
     /* Key bytes are pre-exported in task context (otPlatRadioSetMacKey).
      * Identify which cached buffer to use by comparing the key reference.
      * Jira TZ-2472 */
-    if (a_key_material.mKeyMaterial.mKeyRef == s_previous_key.mKeyMaterial.mKeyRef) {
-        memcpy(s_security_key, s_pervious_key_bytes, 16);
-    } else if (a_key_material.mKeyMaterial.mKeyRef == s_next_key.mKeyMaterial.mKeyRef) {
-        memcpy(s_security_key, s_next_key_bytes, 16);
+    if (a_key_material.mKeyMaterial.mKeyRef == s_sec_ctx[idx].previous_key.mKeyMaterial.mKeyRef) {
+        memcpy(s_sec_ctx[idx].security_key, s_sec_ctx[idx].previous_key_bytes, 16);
+    } else if (a_key_material.mKeyMaterial.mKeyRef == s_sec_ctx[idx].next_key.mKeyMaterial.mKeyRef) {
+        memcpy(s_sec_ctx[idx].security_key, s_sec_ctx[idx].next_key_bytes, 16);
     } else {
-        memcpy(s_security_key, s_current_key_bytes, 16);
+        memcpy(s_sec_ctx[idx].security_key, s_sec_ctx[idx].current_key_bytes, 16);
     }
 #else
-    memcpy(s_security_key, a_key_material.mKeyMaterial.mKey.m8, sizeof(a_key_material.mKeyMaterial.mKey.m8));
+    memcpy(s_sec_ctx[idx].security_key, a_key_material.mKeyMaterial.mKey.m8,
+           sizeof(a_key_material.mKeyMaterial.mKey.m8));
 #endif
 }
-
 #endif // OPENTHREAD_CONFIG_THREAD_VERSION >= OT_THREAD_VERSION_1_2
 
 static esp_openthread_circular_queue_info_t s_recv_queue = {.head = 0, .tail = 0, .used = 0};
 
+static void radio_tx_queue_pop_head(void)
+{
+    assert(s_tx_queue_info.used > 0);
+    s_tx_queue_info.head = (s_tx_queue_info.head + 1) % OT_INSTANCE_COUNT;
+    s_tx_queue_info.used--;
+}
+
+static void radio_start_transmit(otInstance *aInstance, otRadioFrame *aFrame, int8_t idx)
+{
+    esp_ieee802154_set_channel(aFrame->mChannel);
+
+    aFrame->mPsdu[-1] = aFrame->mLength; // length locates one byte before the psdu (esp_openthread_radio_tx_psdu);
+
+    if (otMacFrameIsSecurityEnabled(aFrame) && !aFrame->mInfo.mTxInfo.mIsSecurityProcessed) {
+#if OPENTHREAD_CONFIG_MAC_CSL_RECEIVER_ENABLE
+        if (!s_transmit_frame[idx].mInfo.mTxInfo.mIsARetx || s_csl_period > 0) {
+#else
+        if (!s_transmit_frame[idx].mInfo.mTxInfo.mIsARetx) {
+#endif // OPENTHREAD_CONFIG_MAC_CSL_RECEIVER_ENABLE
+            otMacFrameSetFrameCounter(aFrame, s_sec_ctx[idx].mac_frame_counter++);
+        }
+        if (otMacFrameIsKeyIdMode1(aFrame)) {
+            s_transmit_frame[idx].mInfo.mTxInfo.mAesKey = &s_sec_ctx[idx].current_key;
+            if (!s_transmit_frame[idx].mInfo.mTxInfo.mIsARetx) {
+                otMacFrameSetKeyId(aFrame, s_sec_ctx[idx].key_id);
+            }
+            esp_ieee802154_get_multipan_extended_address((esp_ieee802154_multipan_index_t)idx, s_sec_ctx[idx].security_addr);
+        }
+        ot_set_security_key_from_key_material(idx, s_sec_ctx[idx].current_key);
+        esp_ieee802154_set_transmit_security(&aFrame->mPsdu[-1], s_sec_ctx[idx].security_key,
+                                             s_sec_ctx[idx].security_addr);
+    }
+
+    if (aFrame->mInfo.mTxInfo.mTxDelay != 0) {
+        esp_ieee802154_transmit_at(&aFrame->mPsdu[-1], aFrame->mInfo.mTxInfo.mCsmaCaEnabled,
+                                   (aFrame->mInfo.mTxInfo.mTxDelayBaseTime + aFrame->mInfo.mTxInfo.mTxDelay));
+    } else {
+        esp_ieee802154_transmit(&aFrame->mPsdu[-1], aFrame->mInfo.mTxInfo.mCsmaCaEnabled);
+    }
+
+    otPlatRadioTxStarted(aInstance, aFrame);
+}
+
 static void set_event(uint8_t event)
 {
     uint64_t event_write = event;
@@ -136,15 +212,40 @@ static inline bool get_event(uint8_t event)
 static void ot_radio_receive_done(uint8_t *data, esp_ieee802154_frame_info_t *frame_info);
 static void ot_radio_receive_sfd_done(void);
 static void ot_radio_transmit_done(const uint8_t *frame, const uint8_t *ack,
-                                        esp_ieee802154_frame_info_t *ack_frame_info);
+                                   esp_ieee802154_frame_info_t *ack_frame_info);
 static void ot_radio_transmit_failed(const uint8_t *frame, esp_ieee802154_tx_error_t error);
 static void ot_radio_transmit_sfd_done(uint8_t *frame);
 static void ot_radio_energy_detect_done(int8_t power);
 static esp_err_t ot_radio_enh_ack_generator(uint8_t *frame, esp_ieee802154_frame_info_t *frame_info,
-                                                 uint8_t *enhack_frame);
+                                            uint8_t *enhack_frame);
+
+static void radio_init_software_context(void)
+{
+    for (int idx = 0; idx < OT_INSTANCE_COUNT; idx++) {
+        s_transmit_frame[idx].mPsdu = s_transmit_psdu[idx].psdu;
+    }
+
+    for (uint8_t i = 0; i < CONFIG_IEEE802154_RX_BUFFER_SIZE; i++) {
+        s_receive_frame[i].frame.mPsdu = NULL;
+    }
+
+    s_ack_frame.mPsdu = NULL;
+    memset(&s_recv_queue, 0, sizeof(esp_openthread_circular_queue_info_t));
+    memset(&s_tx_queue_info, 0, sizeof(esp_openthread_tx_queue_info_t));
+    s_txrx_events = 0;
+
+#if OPENTHREAD_CONFIG_MAC_HEADER_IE_SUPPORT
+    for (int idx = 0; idx < OT_INSTANCE_COUNT; idx++) {
+        s_transmit_frame[idx].mInfo.mTxInfo.mIeInfo = &s_transmit_ie_info[idx];
+    }
+#endif
+}
 
 esp_err_t esp_openthread_radio_init(const esp_openthread_platform_config_t *config)
 {
+    esp_err_t ret = ESP_OK;
+    const uint8_t multipan_mask = (uint8_t)((1U << CONFIG_IEEE802154_INTERFACE_NUM) - 1U);
+
     ESP_RETURN_ON_FALSE(s_radio_event_fd == -1, ESP_ERR_INVALID_STATE, OT_PLAT_LOG_TAG,
                         "Radio was initialized already!");
 
@@ -161,26 +262,37 @@ esp_err_t esp_openthread_radio_init(const esp_openthread_platform_config_t *conf
                         "Failed to register ieee802154 event callbacks");
 
     s_radio_event_fd = eventfd(0, EFD_SUPPORT_ISR);
+    ESP_GOTO_ON_FALSE(s_radio_event_fd != -1, ESP_FAIL, exit_unregister, OT_PLAT_LOG_TAG, "Failed to create eventfd");
 
-    s_transmit_frame.mPsdu = s_transmit_psdu.psdu;
-
-    for (uint8_t i = 0; i < CONFIG_IEEE802154_RX_BUFFER_SIZE; i++) {
-        s_receive_frame[i].mPsdu = NULL;
-    }
-
-    s_ack_frame.mPsdu = NULL;
-    memset(&s_recv_queue, 0, sizeof(esp_openthread_circular_queue_info_t));
-
-#if OPENTHREAD_CONFIG_MAC_HEADER_IE_SUPPORT
-    s_transmit_frame.mInfo.mTxInfo.mIeInfo = &s_transmit_ie_info;
-#endif
+    radio_init_software_context();
 
     esp_ieee802154_enable();
-    esp_ieee802154_set_promiscuous(false);
-    esp_ieee802154_set_rx_when_idle(true);
+    ESP_GOTO_ON_ERROR(esp_ieee802154_set_multipan_enable(multipan_mask), exit, OT_PLAT_LOG_TAG,
+                      "Fail to set multipan mask");
 
-    return esp_openthread_platform_workflow_register(&esp_openthread_radio_update, &esp_openthread_radio_process,
-                                                     s_radio_workflow);
+    for (int i = 0; i < CONFIG_IEEE802154_INTERFACE_NUM; i++) {
+        if (multipan_mask & (1U << i)) {
+            esp_ieee802154_multipan_set_rx_when_idle(i, true);
+        }
+    }
+    esp_ieee802154_set_promiscuous(false);
+
+    ESP_GOTO_ON_ERROR(esp_openthread_platform_workflow_register(&esp_openthread_radio_update,
+                                                                &esp_openthread_radio_process,
+                                                                s_radio_workflow),
+                      exit, OT_PLAT_LOG_TAG, "Fail to register radio workflow");
+
+    return ESP_OK;
+
+exit:
+    esp_ieee802154_disable();
+    if (s_radio_event_fd != -1) {
+        close(s_radio_event_fd);
+        s_radio_event_fd = -1;
+    }
+exit_unregister:
+    esp_ieee802154_event_callback_list_unregister();
+    return ret;
 }
 
 void esp_openthread_radio_deinit(void)
@@ -190,6 +302,10 @@ void esp_openthread_radio_deinit(void)
         s_radio_event_fd = -1;
     }
 
+    memset(&s_recv_queue, 0, sizeof(esp_openthread_circular_queue_info_t));
+    memset(&s_tx_queue_info, 0, sizeof(esp_openthread_tx_queue_info_t));
+    s_txrx_events = 0;
+
     esp_ieee802154_disable();
     esp_ieee802154_event_callback_list_unregister();
     esp_openthread_platform_workflow_unregister(s_radio_workflow);
@@ -208,21 +324,32 @@ esp_err_t esp_openthread_radio_process(otInstance *aInstance, const esp_openthre
     uint64_t event_read;
     int ret = read(s_radio_event_fd, &event_read, sizeof(event_read));
     assert(ret == sizeof(event_read));
+    bool start_pending_tx = false;
 
     if (get_event(EVENT_TX_DONE)) {
         clr_event(EVENT_TX_DONE);
+        assert(s_tx_queue_info.used > 0);
+        int8_t tx_idx = s_tx_queue[s_tx_queue_info.head].index;
+        assert(tx_idx >= 0 && tx_idx < OT_INSTANCE_COUNT);
+        otInstance *tx_instance = esp_openthread_get_instance_from_idx(tx_idx);
 
         if (s_ack_frame.mPsdu == NULL) {
-            otPlatRadioTxDone(aInstance, &s_transmit_frame, NULL, OT_ERROR_NONE);
+            otPlatRadioTxDone(tx_instance, &s_transmit_frame[tx_idx], NULL, OT_ERROR_NONE);
         } else {
-            otPlatRadioTxDone(aInstance, &s_transmit_frame, &s_ack_frame, OT_ERROR_NONE);
+            otPlatRadioTxDone(tx_instance, &s_transmit_frame[tx_idx], &s_ack_frame, OT_ERROR_NONE);
             esp_ieee802154_receive_handle_done(s_ack_frame.mPsdu - 1);
             s_ack_frame.mPsdu = NULL;
         }
+        radio_tx_queue_pop_head();
+        start_pending_tx = true;
     }
 
     if (get_event(EVENT_TX_FAILED)) {
         clr_event(EVENT_TX_FAILED);
+        assert(s_tx_queue_info.used > 0);
+        int8_t tx_idx = s_tx_queue[s_tx_queue_info.head].index;
+        assert(tx_idx >= 0 && tx_idx < OT_INSTANCE_COUNT);
+        otInstance *tx_instance = esp_openthread_get_instance_from_idx(tx_idx);
 
         otError err = OT_ERROR_NONE;
 
@@ -243,7 +370,17 @@ esp_err_t esp_openthread_radio_process(otInstance *aInstance, const esp_openthre
             break;
         }
 
-        otPlatRadioTxDone(aInstance, &s_transmit_frame, NULL, err);
+        otPlatRadioTxDone(tx_instance, &s_transmit_frame[tx_idx], NULL, err);
+        radio_tx_queue_pop_head();
+        start_pending_tx = true;
+    }
+
+    // After in-flight TX finishes, start the next frame already in the queue
+    // before processing RX, to reduce TX idle time.
+    if (start_pending_tx && s_tx_queue_info.used > 0) {
+        esp_openthread_radio_transmit_context_t *packet = &s_tx_queue[s_tx_queue_info.head];
+        otInstance *instance = esp_openthread_get_instance_from_idx(packet->index);
+        radio_start_transmit(instance, packet->aFrame, packet->index);
     }
 
     if (get_event(EVENT_ENERGY_DETECT_DONE)) {
@@ -252,10 +389,20 @@ esp_err_t esp_openthread_radio_process(otInstance *aInstance, const esp_openthre
     }
 
     while (atomic_load(&s_recv_queue.used)) {
-        if (s_receive_frame[s_recv_queue.head].mPsdu != NULL) {
-            otPlatRadioReceiveDone(aInstance, &s_receive_frame[s_recv_queue.head], OT_ERROR_NONE);
-            esp_ieee802154_receive_handle_done(s_receive_frame[s_recv_queue.head].mPsdu - 1);
-            s_receive_frame[s_recv_queue.head].mPsdu = NULL;
+        if (s_receive_frame[s_recv_queue.head].frame.mPsdu != NULL) {
+            if (s_receive_frame[s_recv_queue.head].index == ESP_IEEE802154_MULTIPAN_MAX) {
+                // should check all used instances and receive packets for multicast packet.
+                // TZ-2585
+                for (int i = 0; i < OT_INSTANCE_COUNT; i++) {
+                    otInstance *ins = esp_openthread_get_instance_from_idx(i);
+                    otPlatRadioReceiveDone(ins, &s_receive_frame[s_recv_queue.head].frame, OT_ERROR_NONE);
+                }
+            } else {
+                otInstance *ins = esp_openthread_get_instance_from_idx(s_receive_frame[s_recv_queue.head].index);
+                otPlatRadioReceiveDone(ins, &s_receive_frame[s_recv_queue.head].frame, OT_ERROR_NONE);
+            }
+            esp_ieee802154_receive_handle_done(s_receive_frame[s_recv_queue.head].frame.mPsdu - 1);
+            s_receive_frame[s_recv_queue.head].frame.mPsdu = NULL;
             s_recv_queue.head = (s_recv_queue.head + 1) % CONFIG_IEEE802154_RX_BUFFER_SIZE;
             atomic_fetch_sub(&s_recv_queue.used, 1);
         }
@@ -278,17 +425,20 @@ void otPlatRadioGetIeeeEui64(otInstance *aInstance, uint8_t *aIeeeEui64)
 
 void otPlatRadioSetPanId(otInstance *aInstance, uint16_t panid)
 {
-    esp_ieee802154_set_panid(panid);
+    esp_ieee802154_multipan_index_t idx = (esp_ieee802154_multipan_index_t)esp_openthread_get_idx_from_instance(aInstance);
+    esp_ieee802154_set_multipan_panid(idx, panid);
 }
 
 void otPlatRadioSetExtendedAddress(otInstance *aInstance, const otExtAddress *aAddress)
 {
-    esp_ieee802154_set_extended_address(aAddress->m8);
+    esp_ieee802154_multipan_index_t idx = (esp_ieee802154_multipan_index_t)esp_openthread_get_idx_from_instance(aInstance);
+    esp_ieee802154_set_multipan_extended_address(idx, aAddress->m8);
 }
 
 void otPlatRadioSetShortAddress(otInstance *aInstance, uint16_t aAddress)
 {
-    esp_ieee802154_set_short_address(aAddress);
+    esp_ieee802154_multipan_index_t idx = (esp_ieee802154_multipan_index_t)esp_openthread_get_idx_from_instance(aInstance);
+    esp_ieee802154_set_multipan_short_address(idx, aAddress);
 }
 
 void otPlatRadioSetPromiscuous(otInstance *aInstance, bool aEnable)
@@ -317,60 +467,47 @@ otError otPlatRadioDisable(otInstance *aInstance)
 
 otError otPlatRadioSleep(otInstance *aInstance)
 {
-    esp_ieee802154_sleep();
+    int8_t idx = esp_openthread_get_idx_from_instance(aInstance);
+    esp_ieee802154_multipan_sleep(idx);
 
     return OT_ERROR_NONE;
 }
 
 otError otPlatRadioReceive(otInstance *aInstance, uint8_t aChannel)
 {
+    int8_t idx = esp_openthread_get_idx_from_instance(aInstance);
     esp_ieee802154_set_channel(aChannel);
-    esp_ieee802154_receive();
+    esp_ieee802154_multipan_receive(idx);
 
     return OT_ERROR_NONE;
 }
 
 otError otPlatRadioTransmit(otInstance *aInstance, otRadioFrame *aFrame)
 {
-    esp_ieee802154_set_channel(aFrame->mChannel);
+    int8_t idx = esp_openthread_get_idx_from_instance(aInstance);
 
-    aFrame->mPsdu[-1] = aFrame->mLength; // length locates one byte before the psdu (esp_openthread_radio_tx_psdu);
+    // One in-flight + pending entries share this queue; under normal OT scheduling
+    // there is at most one outstanding TX per instance.
+    assert(s_tx_queue_info.used < OT_INSTANCE_COUNT);
 
-    if (otMacFrameIsSecurityEnabled(aFrame) && !aFrame->mInfo.mTxInfo.mIsSecurityProcessed) {
-#if OPENTHREAD_CONFIG_MAC_CSL_RECEIVER_ENABLE
-        if (!s_transmit_frame.mInfo.mTxInfo.mIsARetx || s_csl_period > 0) {
-#else
-        if (!s_transmit_frame.mInfo.mTxInfo.mIsARetx) {
-#endif // OPENTHREAD_CONFIG_MAC_CSL_RECEIVER_ENABLE
-            otMacFrameSetFrameCounter(aFrame, s_mac_frame_counter++);
-        }
-        if (otMacFrameIsKeyIdMode1(aFrame)) {
-            s_transmit_frame.mInfo.mTxInfo.mAesKey = &s_current_key;
-            if (!s_transmit_frame.mInfo.mTxInfo.mIsARetx) {
-                otMacFrameSetKeyId(aFrame, s_key_id);
-            }
-            esp_ieee802154_get_extended_address(s_security_addr);
-        }
-        ot_set_security_key_from_key_material(s_current_key);
-        esp_ieee802154_set_transmit_security(&aFrame->mPsdu[-1], s_security_key, s_security_addr);
+    bool was_idle = (s_tx_queue_info.used == 0);
+    esp_openthread_radio_transmit_context_t *packet = &s_tx_queue[s_tx_queue_info.tail];
+    packet->aFrame = aFrame;
+    packet->index = idx;
+    s_tx_queue_info.tail = (s_tx_queue_info.tail + 1) % OT_INSTANCE_COUNT;
+    s_tx_queue_info.used++;
+
+    if (was_idle) {
+        radio_start_transmit(aInstance, aFrame, idx);
     }
 
-    if (aFrame->mInfo.mTxInfo.mTxDelay != 0) {
-        esp_ieee802154_transmit_at(&aFrame->mPsdu[-1], aFrame->mInfo.mTxInfo.mCsmaCaEnabled,
-                                   (aFrame->mInfo.mTxInfo.mTxDelayBaseTime + aFrame->mInfo.mTxInfo.mTxDelay));
-    } else
-    {
-        esp_ieee802154_transmit(&aFrame->mPsdu[-1], aFrame->mInfo.mTxInfo.mCsmaCaEnabled);
-    }
-
-    otPlatRadioTxStarted(aInstance, aFrame);
-
     return OT_ERROR_NONE;
 }
 
 otRadioFrame *otPlatRadioGetTransmitBuffer(otInstance *aInstance)
 {
-    return &s_transmit_frame;
+    int8_t idx = esp_openthread_get_idx_from_instance(aInstance);
+    return &s_transmit_frame[idx];
 }
 
 int8_t otPlatRadioGetRssi(otInstance *aInstance)
@@ -391,6 +528,7 @@ otRadioCaps otPlatRadioGetCaps(otInstance *aInstance)
 
 otError otPlatRadioReceiveAt(otInstance *aInstance, uint8_t aChannel, uint32_t aStart, uint32_t aDuration)
 {
+    // Multipan RCP does not support receive_at yet.
     esp_ieee802154_set_channel(aChannel);
     esp_ieee802154_receive_at(aStart, aDuration);
     return OT_ERROR_NONE;
@@ -412,40 +550,47 @@ void otPlatRadioEnableSrcMatch(otInstance *aInstance, bool aEnable)
 
 otError otPlatRadioAddSrcMatchShortEntry(otInstance *aInstance, uint16_t aShortAddress)
 {
-    esp_ieee802154_add_pending_addr((uint8_t *)&aShortAddress, true);
+    int8_t idx = esp_openthread_get_idx_from_instance(aInstance);
+    esp_ieee802154_multipan_add_pending_addr(idx, (uint8_t *)&aShortAddress, true);
     return OT_ERROR_NONE;
 }
 
 otError otPlatRadioAddSrcMatchExtEntry(otInstance *aInstance, const otExtAddress *aExtAddress)
 {
-    esp_ieee802154_add_pending_addr(aExtAddress->m8, false);
+    int8_t idx = esp_openthread_get_idx_from_instance(aInstance);
+    esp_ieee802154_multipan_add_pending_addr(idx, aExtAddress->m8, false);
     return OT_ERROR_NONE;
 }
 
 otError otPlatRadioClearSrcMatchShortEntry(otInstance *aInstance, uint16_t aShortAddress)
 {
-    esp_ieee802154_clear_pending_addr((uint8_t *)&aShortAddress, true);
+    int8_t idx = esp_openthread_get_idx_from_instance(aInstance);
+    esp_ieee802154_multipan_clear_pending_addr(idx, (uint8_t *)&aShortAddress, true);
     return OT_ERROR_NONE;
 }
 
 otError otPlatRadioClearSrcMatchExtEntry(otInstance *aInstance, const otExtAddress *aExtAddress)
 {
-    esp_ieee802154_clear_pending_addr(aExtAddress->m8, false);
+    int8_t idx = esp_openthread_get_idx_from_instance(aInstance);
+    esp_ieee802154_multipan_clear_pending_addr(idx, aExtAddress->m8, false);
     return OT_ERROR_NONE;
 }
 
 void otPlatRadioClearSrcMatchShortEntries(otInstance *aInstance)
 {
-    esp_ieee802154_reset_pending_table(true);
+    int8_t idx = esp_openthread_get_idx_from_instance(aInstance);
+    esp_ieee802154_multipan_reset_pending_table(idx, true);
 }
 
 void otPlatRadioClearSrcMatchExtEntries(otInstance *aInstance)
 {
-    esp_ieee802154_reset_pending_table(false);
+    int8_t idx = esp_openthread_get_idx_from_instance(aInstance);
+    esp_ieee802154_multipan_reset_pending_table(idx, false);
 }
 
 otError otPlatRadioEnergyScan(otInstance *aInstance, uint8_t aScanChannel, uint16_t aScanDuration)
 {
+    // TZ-2587
     esp_ieee802154_set_channel(aScanChannel);
     esp_ieee802154_energy_detect(aScanDuration * US_PER_MS / US_PER_SYMBOL);
 
@@ -532,7 +677,6 @@ void otPlatDiagAlarmCallback(otInstance *aInstance)
 void otPlatRadioSetMacKey(otInstance *aInstance, uint8_t aKeyIdMode, uint8_t aKeyId, const otMacKeyMaterial *aPrevKey,
                           const otMacKeyMaterial *aCurrKey, const otMacKeyMaterial *aNextKey, otRadioKeyType aKeyType)
 {
-    OT_UNUSED_VARIABLE(aInstance);
     OT_UNUSED_VARIABLE(aKeyIdMode);
 #if OPENTHREAD_CONFIG_PLATFORM_KEY_REFERENCES_ENABLE
     assert(aKeyType == OT_KEY_TYPE_KEY_REF);
@@ -541,33 +685,33 @@ void otPlatRadioSetMacKey(otInstance *aInstance, uint8_t aKeyIdMode, uint8_t aKe
 #endif // OPENTHREAD_CONFIG_PLATFORM_KEY_REFERENCES_ENABLE
     assert(aPrevKey != NULL && aCurrKey != NULL && aNextKey != NULL);
 
-    s_key_id = aKeyId;
-    s_previous_key = *aPrevKey;
-    s_current_key  = *aCurrKey;
-    s_next_key     = *aNextKey;
+    int8_t idx = esp_openthread_get_idx_from_instance(aInstance);
+
+    s_sec_ctx[idx].key_id = aKeyId;
+    s_sec_ctx[idx].previous_key = *aPrevKey;
+    s_sec_ctx[idx].current_key  = *aCurrKey;
+    s_sec_ctx[idx].next_key     = *aNextKey;
 #if OPENTHREAD_CONFIG_PLATFORM_KEY_REFERENCES_ENABLE
     /* Pre-export raw key bytes in task context to avoid calling psa_export_key()
      * from ISR context (enh_ack_generator), which would attempt to take a mutex. */
     size_t keyLength = 0;
-    psa_export_key(aPrevKey->mKeyMaterial.mKeyRef, s_pervious_key_bytes, 16, &keyLength);
-    psa_export_key(aCurrKey->mKeyMaterial.mKeyRef, s_current_key_bytes,  16, &keyLength);
-    psa_export_key(aNextKey->mKeyMaterial.mKeyRef, s_next_key_bytes,     16, &keyLength);
+    psa_export_key(aPrevKey->mKeyMaterial.mKeyRef, s_sec_ctx[idx].previous_key_bytes, 16, &keyLength);
+    psa_export_key(aCurrKey->mKeyMaterial.mKeyRef, s_sec_ctx[idx].current_key_bytes,  16, &keyLength);
+    psa_export_key(aNextKey->mKeyMaterial.mKeyRef, s_sec_ctx[idx].next_key_bytes,     16, &keyLength);
 #endif
 }
 
 void otPlatRadioSetMacFrameCounter(otInstance *aInstance, uint32_t aMacFrameCounter)
 {
-    OT_UNUSED_VARIABLE(aInstance);
-
-    s_mac_frame_counter = aMacFrameCounter;
+    int8_t idx = esp_openthread_get_idx_from_instance(aInstance);
+    s_sec_ctx[idx].mac_frame_counter = aMacFrameCounter;
 }
 
 void otPlatRadioSetMacFrameCounterIfLarger(otInstance *aInstance, uint32_t aMacFrameCounter)
 {
-    OT_UNUSED_VARIABLE(aInstance);
-
-    if (aMacFrameCounter > s_mac_frame_counter) {
-        s_mac_frame_counter = aMacFrameCounter;
+    int8_t idx = esp_openthread_get_idx_from_instance(aInstance);
+    if (aMacFrameCounter > s_sec_ctx[idx].mac_frame_counter) {
+        s_sec_ctx[idx].mac_frame_counter = aMacFrameCounter;
     }
 }
 #endif // OPENTHREAD_CONFIG_THREAD_VERSION >= OT_THREAD_VERSION_1_2
@@ -635,9 +779,11 @@ uint8_t otPlatRadioGetCslUncertainty(otInstance *aInstance)
 
 // events
 static void IRAM_ATTR ot_radio_transmit_done(const uint8_t *frame, const uint8_t *ack,
-                                                  esp_ieee802154_frame_info_t *ack_frame_info)
+                                             esp_ieee802154_frame_info_t *ack_frame_info)
 {
-    ETS_ASSERT(frame == (uint8_t *)&s_transmit_psdu);
+    int8_t tx_idx = s_tx_queue[s_tx_queue_info.head].index;
+    ETS_ASSERT(tx_idx >= 0 && tx_idx < OT_INSTANCE_COUNT);
+    ETS_ASSERT(frame == (uint8_t *)&s_transmit_psdu[tx_idx]);
 
     if (ack != NULL) {
         s_ack_frame.mLength = (uint16_t)(*ack);
@@ -663,40 +809,48 @@ static void IRAM_ATTR convert_to_ot_frame(uint8_t *data, esp_ieee802154_frame_in
     radio_frame->mInfo.mRxInfo.mTimestamp = frame_info->timestamp;
 }
 
-static esp_err_t IRAM_ATTR enh_ack_set_security_addr_and_key(otRadioFrame *ack_frame)
+static esp_err_t IRAM_ATTR enh_ack_set_security_addr_and_key(otRadioFrame *ack_frame, int8_t idx)
 {
     struct otMacKeyMaterial *key = NULL;
     uint8_t key_id;
 
     ETS_ASSERT(otMacFrameIsSecurityEnabled(ack_frame));
+    if (idx < 0 || idx >= OT_INSTANCE_COUNT) {
+        return ESP_FAIL;
+    }
     key_id = otMacFrameGetKeyId(ack_frame);
     if(!(otMacFrameIsKeyIdMode1(ack_frame) && key_id != 0)) {
         return ESP_FAIL;
     }
 
-    if (key_id == s_key_id) {
-        key = &s_current_key;
-    } else if (key_id == s_key_id - 1) {
-        key = &s_previous_key;
-    } else if (key_id == s_key_id + 1) {
-        key = &s_next_key;
+    if (key_id == s_sec_ctx[idx].key_id) {
+        key = &s_sec_ctx[idx].current_key;
+    } else if (key_id == s_sec_ctx[idx].key_id - 1) {
+        key = &s_sec_ctx[idx].previous_key;
+    } else if (key_id == s_sec_ctx[idx].key_id + 1) {
+        key = &s_sec_ctx[idx].next_key;
     } else {
         return ESP_FAIL;
     }
-    s_ack_frame_counter = s_mac_frame_counter;
-    s_ack_key_id = key_id;
-    s_with_security_enh_ack = true;
+    s_sec_ctx[idx].ack_frame_counter = s_sec_ctx[idx].mac_frame_counter;
+    s_sec_ctx[idx].ack_key_id = key_id;
+    s_sec_ctx[idx].with_security_enh_ack = true;
     if (otMacFrameIsKeyIdMode1(ack_frame)) {
-        esp_ieee802154_get_extended_address(s_security_addr);
-        ot_set_security_key_from_key_material(*key);
+#if CONFIG_IEEE802154_MULTI_PAN_ENABLE
+        esp_ieee802154_get_multipan_extended_address((esp_ieee802154_multipan_index_t)idx, s_sec_ctx[idx].security_addr);
+#else
+        esp_ieee802154_get_extended_address(s_sec_ctx[idx].security_addr);
+#endif
+        ot_set_security_key_from_key_material(idx, *key);
     }
 
-    esp_ieee802154_set_transmit_security(&ack_frame->mPsdu[-1], s_security_key, s_security_addr);
+    esp_ieee802154_set_transmit_security(&ack_frame->mPsdu[-1], s_sec_ctx[idx].security_key,
+                                         s_sec_ctx[idx].security_addr);
     return ESP_OK;
 }
 
 static esp_err_t IRAM_ATTR ot_radio_enh_ack_generator(uint8_t *frame, esp_ieee802154_frame_info_t *frame_info,
-                                                           uint8_t *enhack_frame)
+                                                      uint8_t *enhack_frame)
 {
     otRadioFrame ack_frame;
     otRadioFrame ot_frame;
@@ -735,8 +889,12 @@ static esp_err_t IRAM_ATTR ot_radio_enh_ack_generator(uint8_t *frame, esp_ieee80
     s_enhack = enhack_frame;
 
     if (otMacFrameIsSecurityEnabled(&ack_frame) && !ack_frame.mInfo.mTxInfo.mIsSecurityProcessed) {
-        otMacFrameSetFrameCounter(&ack_frame, s_mac_frame_counter++);
-        if (enh_ack_set_security_addr_and_key(&ack_frame) != ESP_OK) {
+        int8_t ack_idx = (int8_t)frame_info->mpf_index;
+        if (ack_idx < 0 || ack_idx >= OT_INSTANCE_COUNT) {
+            return ESP_FAIL;
+        }
+        otMacFrameSetFrameCounter(&ack_frame, s_sec_ctx[ack_idx].mac_frame_counter++);
+        if (enh_ack_set_security_addr_and_key(&ack_frame, ack_idx) != ESP_OK) {
             return ESP_FAIL;
         }
     }
@@ -747,6 +905,8 @@ static void IRAM_ATTR ot_radio_receive_done(uint8_t *data, esp_ieee802154_frame_
 {
     otRadioFrame ot_frame;
     ot_frame.mPsdu = data + 1;
+    int8_t rx_idx = (int8_t)frame_info->mpf_index;
+    assert((rx_idx >= 0 && rx_idx < OT_INSTANCE_COUNT) || rx_idx == ESP_IEEE802154_MULTIPAN_MAX);
 
     if (atomic_load(&s_recv_queue.used) == CONFIG_IEEE802154_RX_BUFFER_SIZE) {
         ESP_EARLY_LOGE(OT_PLAT_LOG_TAG, "radio receive buffer full!");
@@ -754,17 +914,20 @@ static void IRAM_ATTR ot_radio_receive_done(uint8_t *data, esp_ieee802154_frame_
         return;
     }
 
-    convert_to_ot_frame(data, frame_info, &(s_receive_frame[s_recv_queue.tail]));
+    convert_to_ot_frame(data, frame_info, &(s_receive_frame[s_recv_queue.tail].frame));
+    s_receive_frame[s_recv_queue.tail].index = rx_idx;
 #if OPENTHREAD_CONFIG_THREAD_VERSION >= OT_THREAD_VERSION_1_2
-    // Inform if this frame was acknowledged with secured Enh-ACK.
-    if (otMacFrameIsAckRequested(&ot_frame) && otMacFrameIsVersion2015(&ot_frame)) {
-        s_receive_frame[s_recv_queue.tail].mInfo.mRxInfo.mAckedWithSecEnhAck = s_with_security_enh_ack;
-        s_receive_frame[s_recv_queue.tail].mInfo.mRxInfo.mAckFrameCounter = s_ack_frame_counter;
-        s_receive_frame[s_recv_queue.tail].mInfo.mRxInfo.mAckKeyId = s_ack_key_id;
-    } else {
-        s_receive_frame[s_recv_queue.tail].mInfo.mRxInfo.mAckedWithSecEnhAck = false;
+    // Default to false for broadcast/unmatched frames or non-2015/non-AR data frames.
+    s_receive_frame[s_recv_queue.tail].frame.mInfo.mRxInfo.mAckedWithSecEnhAck = false;
+    if (rx_idx >= 0 && rx_idx < OT_INSTANCE_COUNT &&
+        otMacFrameIsAckRequested(&ot_frame) && otMacFrameIsVersion2015(&ot_frame)) {
+        s_receive_frame[s_recv_queue.tail].frame.mInfo.mRxInfo.mAckedWithSecEnhAck = s_sec_ctx[rx_idx].with_security_enh_ack;
+        s_receive_frame[s_recv_queue.tail].frame.mInfo.mRxInfo.mAckFrameCounter = s_sec_ctx[rx_idx].ack_frame_counter;
+        s_receive_frame[s_recv_queue.tail].frame.mInfo.mRxInfo.mAckKeyId = s_sec_ctx[rx_idx].ack_key_id;
+    }
+    if (rx_idx >= 0 && rx_idx < OT_INSTANCE_COUNT) {
+        s_sec_ctx[rx_idx].with_security_enh_ack = false;
     }
-    s_with_security_enh_ack = false;
 #endif // OPENTHREAD_CONFIG_THREAD_VERSION >= OT_THREAD_VERSION_1_2
     s_recv_queue.tail = (s_recv_queue.tail + 1) % CONFIG_IEEE802154_RX_BUFFER_SIZE;
     atomic_fetch_add(&s_recv_queue.used, 1);
@@ -773,7 +936,9 @@ static void IRAM_ATTR ot_radio_receive_done(uint8_t *data, esp_ieee802154_frame_
 
 static void IRAM_ATTR ot_radio_transmit_failed(const uint8_t *frame, esp_ieee802154_tx_error_t error)
 {
-    ETS_ASSERT(frame == (uint8_t *)&s_transmit_psdu);
+    int8_t tx_idx = s_tx_queue[s_tx_queue_info.head].index;
+    ETS_ASSERT(tx_idx >= 0 && tx_idx < OT_INSTANCE_COUNT);
+    ETS_ASSERT(frame == (uint8_t *)&s_transmit_psdu[tx_idx]);
 
     s_tx_error = error;
 
@@ -786,7 +951,16 @@ static void IRAM_ATTR ot_radio_receive_sfd_done(void)
 
 static void IRAM_ATTR ot_radio_transmit_sfd_done(uint8_t *frame)
 {
-    assert(frame == (uint8_t *)&s_transmit_psdu || frame == s_enhack);
+    int8_t idx = -1;
+
+    if (frame != s_enhack) {
+        idx = s_tx_queue[s_tx_queue_info.head].index;
+        assert(idx >= 0 && idx < OT_INSTANCE_COUNT);
+        assert(frame == (uint8_t *)&s_transmit_psdu[idx]);
+    }
+#if !OPENTHREAD_CONFIG_TIME_SYNC_ENABLE
+    OT_UNUSED_VARIABLE(idx);
+#endif
 
 #if OPENTHREAD_CONFIG_MAC_CSL_RECEIVER_ENABLE
     if (s_csl_period > 0) {
@@ -799,12 +973,12 @@ static void IRAM_ATTR ot_radio_transmit_sfd_done(uint8_t *frame)
 #endif
 
 #if OPENTHREAD_CONFIG_TIME_SYNC_ENABLE
-    if (frame == (uint8_t *)&s_transmit_psdu && s_transmit_frame.mInfo.mTxInfo.mIeInfo->mTimeIeOffset != 0)
+    if ((idx >= 0) && s_transmit_frame[idx].mInfo.mTxInfo.mIeInfo->mTimeIeOffset != 0)
     {
-        uint8_t *p_time_ie = s_transmit_frame.mPsdu + s_transmit_frame.mInfo.mTxInfo.mIeInfo->mTimeIeOffset;
-        uint64_t time = (uint64_t)((int64_t)otPlatTimeGet() + s_transmit_frame.mInfo.mTxInfo.mIeInfo->mNetworkTimeOffset);
+        uint8_t *p_time_ie = s_transmit_frame[idx].mPsdu + s_transmit_frame[idx].mInfo.mTxInfo.mIeInfo->mTimeIeOffset;
+        uint64_t time = (uint64_t)((int64_t)otPlatTimeGet() + s_transmit_frame[idx].mInfo.mTxInfo.mIeInfo->mNetworkTimeOffset);
 
-        *p_time_ie = s_transmit_frame.mInfo.mTxInfo.mIeInfo->mTimeSyncSeq;
+        *p_time_ie = s_transmit_frame[idx].mInfo.mTxInfo.mIeInfo->mTimeSyncSeq;
 
         *(++p_time_ie) = (uint8_t)(time & 0xff);
         for (uint8_t i = 1; i < sizeof(uint64_t); i++)
@@ -842,8 +1016,8 @@ otError otPlatRadioSetChannelMaxTransmitPower(otInstance *aInstance, uint8_t aCh
 #if CONFIG_OPENTHREAD_RX_ON_WHEN_IDLE
 void otPlatRadioSetRxOnWhenIdle(otInstance *aInstance, bool aEnable)
 {
-    OT_UNUSED_VARIABLE(aInstance);
-    esp_ieee802154_set_rx_when_idle(aEnable);
+    int8_t idx = esp_openthread_get_idx_from_instance(aInstance);
+    esp_ieee802154_multipan_set_rx_when_idle(idx, aEnable);
 }
 #endif
 
@@ -875,3 +1049,16 @@ void esp_ieee802154_receive_at_done(void)
 {
     set_event(EVENT_SLEEP);
 }
+
+otError otPlatMultipanGetActiveInstance(otInstance **aInstance)
+{
+    *aInstance = esp_openthread_get_instance();
+    return OT_ERROR_NONE;
+}
+
+otError otPlatMultipanSetActiveInstance(otInstance *aInstance, bool aCompletePending)
+{
+    OT_UNUSED_VARIABLE(aCompletePending);
+    esp_openthread_set_active_instance(aInstance);
+    return OT_ERROR_NONE;
+}
diff --git a/components/openthread/src/ncp/esp_openthread_ncp.cpp b/components/openthread/src/ncp/esp_openthread_ncp.cpp
index eb0bad9c508..67f87df3eaa 100644
--- a/components/openthread/src/ncp/esp_openthread_ncp.cpp
+++ b/components/openthread/src/ncp/esp_openthread_ncp.cpp
@@ -93,6 +93,31 @@ static esp_err_t init_console_command_worker()
 }
 #endif // CONFIG_OPENTHREAD_RCP_SPINEL_CONSOLE
 
+static void ncp_init_console(void)
+{
+#if CONFIG_OPENTHREAD_RCP_SPINEL_CONSOLE
+    esp_err_t err = esp_console_redirect_to_otlog();
+    if (err != ESP_OK) {
+        ESP_LOGE(NO_LOG_TAG, "Failed to redirect console to otPlatLog: %s", esp_err_to_name(err));
+    }
+
+    init_console_command_worker();
+#endif
+}
+
+#if CONFIG_OPENTHREAD_MULTIPAN_RCP_ENABLE
+extern "C" void otAppNcpInitMulti(otInstance **aInstances, uint8_t aCount)
+{
+#if (CONFIG_OPENTHREAD_RCP_UART || CONFIG_OPENTHREAD_RCP_USB_SERIAL_JTAG || CONFIG_OPENTHREAD_RCP_CUSTOM)
+    IgnoreError(otPlatUartEnable());
+    otNcpHdlcInitMulti(aInstances, aCount, NcpSend);
+#else
+#error "Multiple instances based on SPI are not supported yet"
+#endif
+
+    ncp_init_console();
+}
+#else
 extern "C" void otAppNcpInit(otInstance *aInstance)
 {
 #if (CONFIG_OPENTHREAD_RCP_UART || CONFIG_OPENTHREAD_RCP_USB_SERIAL_JTAG)
@@ -102,15 +127,9 @@ extern "C" void otAppNcpInit(otInstance *aInstance)
     otNcpSpiInit(aInstance);
 #endif
 
-#if CONFIG_OPENTHREAD_RCP_SPINEL_CONSOLE
-    esp_err_t err = esp_console_redirect_to_otlog();
-    if (err != ESP_OK) {
-        ESP_LOGE(NO_LOG_TAG, "Failed to redirect console to otPlatLog: %s", esp_err_to_name(err));
-    }
-
-    init_console_command_worker();
-#endif // CONFIG_OPENTHREAD_RCP_SPINEL_CONSOLE
+    ncp_init_console();
 }
+#endif
 
 namespace ot {
 namespace Ncp {
diff --git a/components/openthread/src/ncp/esp_openthread_ncp_hdlc.cpp b/components/openthread/src/ncp/esp_openthread_ncp_hdlc.cpp
index 6b86b6d9359..db8cd395acb 100644
--- a/components/openthread/src/ncp/esp_openthread_ncp_hdlc.cpp
+++ b/components/openthread/src/ncp/esp_openthread_ncp_hdlc.cpp
@@ -1,9 +1,10 @@
 /*
- * SPDX-FileCopyrightText: 2023-2024 Espressif Systems (Shanghai) CO LTD
+ * SPDX-FileCopyrightText: 2023-2026 Espressif Systems (Shanghai) CO LTD
  *
  * SPDX-License-Identifier: Apache-2.0
  */
 
+#include "sdkconfig.h"
 #include "common/new.hpp"
 #include "ncp_hdlc.hpp"
 
@@ -12,6 +13,30 @@ namespace Ncp {
 
 static OT_DEFINE_ALIGNED_VAR(sNcpRaw, sizeof(NcpHdlc), uint64_t);
 
+#if CONFIG_OPENTHREAD_MULTIPAN_RCP_ENABLE
+extern "C" void otNcpHdlcInitMulti(otInstance **aInstances, uint8_t aCount, otNcpHdlcSendCallback aSendCallback)
+{
+    NcpHdlc      *ncpHdlc = nullptr;
+    ot::Instance *instances[SPINEL_HEADER_IID_MAX];
+
+    OT_ASSERT(aCount < SPINEL_HEADER_IID_MAX + 1);
+    OT_ASSERT(aCount > 0);
+    OT_ASSERT(aInstances[0] != nullptr);
+
+    for (int i = 0; i < aCount; i++)
+    {
+        OT_ASSERT(aInstances[i] != nullptr);
+        instances[i] = static_cast<ot::Instance *>(aInstances[i]);
+    }
+
+    ncpHdlc = new (&sNcpRaw) NcpHdlc(instances, aCount, aSendCallback);
+
+    if (ncpHdlc == nullptr || ncpHdlc != NcpBase::GetNcpInstance())
+    {
+        OT_ASSERT(false);
+    }
+}
+#else
 extern "C" void otNcpHdlcInit(otInstance *aInstance, otNcpHdlcSendCallback aSendCallback)
 {
     NcpHdlc  *ncpHdlc  = nullptr;
@@ -24,6 +49,7 @@ extern "C" void otNcpHdlcInit(otInstance *aInstance, otNcpHdlcSendCallback aSend
         OT_ASSERT(false);
     }
 }
+#endif
 
 } // namespace Ncp
 } // namespace ot
diff --git a/components/openthread/srcs_radio.cmake b/components/openthread/srcs_radio.cmake
index 0fc4756d5a2..c9fdf754991 100644
--- a/components/openthread/srcs_radio.cmake
+++ b/components/openthread/srcs_radio.cmake
@@ -60,18 +60,31 @@ set(rcp_srcs
     openthread/src/ncp/ncp_base_dispatcher.cpp
     openthread/src/ncp/ncp_base_radio.cpp
     openthread/examples/apps/ncp/ncp.c
-    # ESP port
-    src/port/esp_openthread_alarm.c
+    # ESP port (logging/misc/settings stay at the original path)
     src/port/esp_openthread_logging.c
     src/port/esp_openthread_misc.c
-    src/port/esp_openthread_radio.c
     src/port/esp_openthread_settings.c
     # ESP sources
     src/esp_openthread.cpp
     src/esp_openthread_lock.c
     src/esp_openthread_platform.cpp
     src/esp_openthread_task_queue.c
-    # ESP NCP
+)
+
+# Single-instance files stay at the original src/ paths. Multipan RCP swaps in
+# radio/alarm/instance under src/multiple_instances/. NCP is shared in src/ncp/.
+if(CONFIG_OPENTHREAD_MULTIPAN_RCP_ENABLE)
+    set(OT_PORT_DIR "src/multiple_instances/port")
+    set(OT_INSTANCE_SRC "src/multiple_instances/esp_openthread_instance.cpp")
+else()
+    set(OT_PORT_DIR "src/port")
+    set(OT_INSTANCE_SRC "src/esp_openthread_instance.cpp")
+endif()
+
+list(APPEND rcp_srcs
+    ${OT_PORT_DIR}/esp_openthread_alarm.c
+    ${OT_PORT_DIR}/esp_openthread_radio.c
+    ${OT_INSTANCE_SRC}
     src/ncp/esp_openthread_ncp.cpp
 )
 
@@ -87,7 +100,9 @@ elseif(CONFIG_OPENTHREAD_RCP_SPI)
     list(APPEND rcp_srcs
         openthread/src/ncp/ncp_spi.cpp
         src/port/esp_openthread_spi_slave.c)
-    if(CONFIG_OPENTHREAD_NCP_VENDOR_HOOK)
+    # Multipan SPI NCP is not supported; the compile-time check lives in
+    # src/ncp/esp_openthread_ncp.cpp.
+    if(CONFIG_OPENTHREAD_NCP_VENDOR_HOOK AND NOT CONFIG_OPENTHREAD_MULTIPAN_RCP_ENABLE)
         list(APPEND rcp_srcs src/ncp/esp_openthread_ncp_spi.cpp)
     endif()
 endif()
diff --git a/examples/openthread/ot_rcp/sdkconfig.ci.multipan b/examples/openthread/ot_rcp/sdkconfig.ci.multipan
new file mode 100644
index 00000000000..2a5ee0a278c
--- /dev/null
+++ b/examples/openthread/ot_rcp/sdkconfig.ci.multipan
@@ -0,0 +1,3 @@
+CONFIG_IEEE802154_MULTI_PAN_ENABLE=y
+CONFIG_OPENTHREAD_MULTIPAN_RCP_ENABLE=y
+CONFIG_OPENTHREAD_MULTIPLE_INTERFACES_COUNT=2